🧬 GenomeInsight

A Python-based bioinformatics toolkit for DNA sequence analysis using the BRCA1 (NM_007294.4) reference sequence.

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📖 Overview

GenomeInsight is a modular bioinformatics project developed in Python to perform fundamental DNA sequence analyses. It demonstrates practical bioinformatics workflows using the BRCA1 gene as a reference sequence.

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✨ Features

• 📄 Read FASTA files
• 📏 Calculate sequence length
• 🔢 Count nucleotides (A, T, G, C)
• 🧪 Calculate GC content
• 🔄 Generate reverse complement
• 🧬 Translate DNA into protein
• 🔍 Search DNA motifs
• 🧩 Find Open Reading Frames (ORFs)
• 📊 Perform k-mer frequency analysis
• 📈 Generate visualizations
  • Nucleotide composition bar chart
  • Nucleotide composition pie chart
  • GC vs AT content chart
• 📋 Generate sequence statistics
• 💾 Export analysis reports

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🛠️ Technologies Used

• Python 3
• Biopython
• Matplotlib
• Git
• GitHub
• PyCharm

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📂 Project Structure

GenomeInsight/
│
├── data/
│   └── sample.fasta
│
├── output/
│   ├── gc_at_chart.png
│   ├── nucleotide_composition.png
│   ├── nucleotide_piechart.png
│   ├── statistics.txt
│   └── results.txt
│
├── src/
│   ├── main.py
│   ├── utils.py
│   ├── read_fasta.py
│   ├── sequence_length.py
│   ├── nucleotide_count.py
│   ├── gc_content.py
│   ├── reverse_complement.py
│   ├── translate_dna.py
│   ├── motif_search.py
│   ├── orf_finder.py
│   ├── kmer_analysis.py
│   ├── export_results.py
│   ├── visualization.py
│   ├── pie_chart.py
│   ├── gc_at_chart.py
│   └── statistics_dashboard.py
│
├── README.md
├── requirements.txt
└── .gitignore

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📊 Dataset

Reference Sequence

• Gene: BRCA1
• Accession: NM_007294.4
• Organism: Homo sapiens

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🚀 Installation

Clone the repository:

git clone https://github.com/<your-username>/GenomeInsight.git

Install dependencies:

pip install -r requirements.txt

Run the project:

python src/main.py

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📸 Sample Output

The toolkit generates:

• Sequence statistics
• Nucleotide composition charts
• GC vs AT visualization
• Exported analysis reports

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📸 Screenshots

GC vs AT Content

Nucleotide Composition (Bar Chart)

Nucleotide Composition (Pie Chart)

🔮 Future Enhancements

• Multiple FASTA sequence support
• Pairwise sequence alignment
• BLAST integration
• Streamlit web interface
• Protein sequence analysis

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👩‍💻 Author

Abha

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📄 License

This project is licensed under the MIT License.