I'm recording from somatostatin neurons that are not so abundant in the Region on the recording, I was able to record approximately 10-15 neurons. but when I run the processing for the proc files and look at the ROIs using the MATLAB GUI, I notice that I get a lot of unnecessary ROIs that look like noise. I tried changing the channel gain and laser power during the recordings. Yet it didn't help the classifier to accurately separate the neurons from the surrounding. so I was wondering if anyone faced similar problem and if there is a way to fix this (maybe changing some of the preprocessing parameters).
I'm recording from somatostatin neurons that are not so abundant in the Region on the recording, I was able to record approximately 10-15 neurons. but when I run the processing for the proc files and look at the ROIs using the MATLAB GUI, I notice that I get a lot of unnecessary ROIs that look like noise. I tried changing the channel gain and laser power during the recordings. Yet it didn't help the classifier to accurately separate the neurons from the surrounding. so I was wondering if anyone faced similar problem and if there is a way to fix this (maybe changing some of the preprocessing parameters).
