diff --git a/packages/react-native/src/private/webapis/intersectionobserver/IntersectionObserver.js b/packages/react-native/src/private/webapis/intersectionobserver/IntersectionObserver.js
index 2d723e8cd7ae..f75e7c0375a7 100644
--- a/packages/react-native/src/private/webapis/intersectionobserver/IntersectionObserver.js
+++ b/packages/react-native/src/private/webapis/intersectionobserver/IntersectionObserver.js
@@ -323,7 +323,7 @@ function normalizeThreshold(
       return threshold
         .map(t => normalizeThresholdValue(t, 'threshold'))
         .map(t => t ?? 0)
-        .sort();
+        .sort((a, b) => a - b);
     } else if (defaultEmpty) {
       return [];
     } else {
@@ -359,7 +359,7 @@ function normalizeRootThreshold(
     const normalizedArr = rootThreshold
       .map(rt => normalizeThresholdValue(rt, 'rnRootThreshold'))
       .filter((rt): rt is number => rt != null)
-      .sort();
+      .sort((a, b) => a - b);
     return normalizedArr.length === 0 ? null : normalizedArr;
   }
 
diff --git a/packages/react-native/src/private/webapis/intersectionobserver/__tests__/IntersectionObserver-itest.js b/packages/react-native/src/private/webapis/intersectionobserver/__tests__/IntersectionObserver-itest.js
index ac6eda8c618a..db5ad0a407b5 100644
--- a/packages/react-native/src/private/webapis/intersectionobserver/__tests__/IntersectionObserver-itest.js
+++ b/packages/react-native/src/private/webapis/intersectionobserver/__tests__/IntersectionObserver-itest.js
@@ -321,6 +321,10 @@ describe('IntersectionObserver', () => {
       expect(
         new IntersectionObserver(() => {}, {threshold: [0.5, 0, 1]}).thresholds,
       ).toEqual([0, 0.5, 1]);
+      expect(
+        new IntersectionObserver(() => {}, {threshold: [0.000001, 1e-7]})
+          .thresholds,
+      ).toEqual([1e-7, 0.000001]);
 
       // Does NOT deduplicate (browsers don't do it - shrug)
       expect(
@@ -427,6 +431,11 @@ describe('IntersectionObserver', () => {
         new IntersectionObserver(() => {}, {rnRootThreshold: [0.5, 0, 1]})
           .rnRootThresholds,
       ).toEqual([0, 0.5, 1]);
+      expect(
+        new IntersectionObserver(() => {}, {
+          rnRootThreshold: [0.000001, 1e-7],
+        }).rnRootThresholds,
+      ).toEqual([1e-7, 0.000001]);
 
       // Does NOT deduplicate (browsers don't do it - shrug)
       expect(